Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe)
Italian health authority and research organization for animal health and food safety
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Diagnostic protocols

After taking up the role as new European Union Reference Laboratory (EURL) for Avian Influenza and Newcastle Disease, starting from January 2019 the Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe) has been committed to making sure that the recommended protocols for the detection, identification and typing of AI and APMV-1 are fit for purpose. This procedure has been carried out by testing the efficacy of the existing methods using newly received viruses or by comparing the performance of the new available methods with the ones in use.

The laboratory protocols which are currently made available by the EURL IZSVe and the former EURL APHA-Weybridge are itemized below. Please contact the corresponding mentioned laboratory if you need any technical advice.

Protocol Laboratory Download
Detection of Avian Influenza virus (AIV) by real-time reverse transcription PCR IZSVe PDF
Avian Influenza virus (AIV) typing by reverse transcription PCR using a universal primer set IZSVe PDF
Detection of avian influenza A virus matrix gene by real time PCR APHA PDF
Detection of avian influenza A viruses of subtype N1 by real time PCR APHA PDF
Detection of avian influenza A viruses of subtype N5 by real time PCR APHA PDF
Detection of avian influenza A viruses of subtype N6, N7 and N9 by real time PCR APHA PDF
Detection of avian influenza A viruses of subtype N8 by real time PCR APHA PDF
Detection of influenza A matrix gene by real time Taqman RT-PCR APHA PDF
Eurasian H5 avian influenza real time PCR APHA PDF
Eurasian H7 avian influenza real time PCR APHA PDF
Extraction of nucleic acid from swabs and processed tissue using the QIAGEN BioRobot Universal APHA PDF
Influenza – Neuraminidase Inhibition Test APHA PDF
Manual nucleic acid extraction from biological samples APHA PDF
One step PCR for detection of H5 and H7 avian influenza virus and cleavage site sequencing APHA PDF
Preparation and processing of swab pools for genetic screening and subtyping of avian influenza A virus and/or Avian Avulavirus type 1 by real time PCR APHA PDF
Procedure for extraction of samples from avian carcasses for virus detection/isolation APHA PDF
Procedure for the isolation and detection of viruses from avian species using embryonated eggs APHA PDF
Receipt and opening of parcels in a AI/ND reference laboratory APHA PDF
Storage of samples and AI/AAvV virus isolates in a reference laboratory APHA PDF
The Intravenous pathogenicity index test for avian influenza APHA PDF
Avian Avulavirus type 1 cleavage site sequencing APHA PDF
Avian Avulavirus type 1 screening real time PCR APHA PDF
Haemagglutination Inhibition tests for Paramyxoviruses (Avulaviruses) and Orthomyxoviruses, Infectious Bronchitis Virus and Egg Drop Syndrome APHA PDF
Molecular pathotyping of Avian Avulavirus type 1 (AAvV-1) APHA PDF
Molecular pathotyping of Avian Avulavirus type 1 by real time PCR APHA PDF
The Intracerebral pathogenicity index test for Avian Avulavirus type 1 APHA PDF

 

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Istituto Zooprofilattico Sperimentale delle Venezie
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